Dr. Sang Hyuck Park, Dr. Eun Soo Kim
This study will provide a base for a future thrust relating to a selection of hemp varieties. According to the morphological aspect, three types of glandular trichomes in Cannabis sativa are recognized: bulbous, capitate-sessile, and capitate-stalked trichomes. Capitate-stalked trichomes have garnered research interest, whereas the other two types of trichomes have almost not been investigated during the past twenty years.
Our goal is to determine the functional activities of the three types of glandular trichomes related to the biosynthesis of cannabinoids. First, we will examine and compare the external features, distribution, and densities of these glandular trichomes using digital microscopy and scanning electron microscopy. Second, we will analyze secretory cavity contents of individual glandular trichomes removed through microcapillary procedures from hundreds trichomes of each type using gas chromatography. Third, we will examine localization and distribution of available antibody probe for CBD in chemically fixed and embedded tissues using transmission electron microscopy.